A Photoactivatable AIE Polymer for Light‐Controlled Gene Delivery: Concurrent Endo/Lysosomal Escape and DNA Unpacking
Identifieur interne : 000F85 ( Main/Exploration ); précédent : 000F84; suivant : 000F86A Photoactivatable AIE Polymer for Light‐Controlled Gene Delivery: Concurrent Endo/Lysosomal Escape and DNA Unpacking
Auteurs : Youyong Yuan [Singapour] ; Chong-Jing Zhang [Singapour] ; Bin Liu [Singapour]Source :
- Angewandte Chemie [ 0044-8249 ] ; 2015-09-21.
Abstract
Endo/lysosomal escape of gene vectors and the subsequent unpacking of nucleic acids in cytosol are two major challenges for efficient gene delivery. Herein, we report a polymeric gene delivery vector, which consists of a photosensitizer (PS) with aggregation‐induced emission (AIE) characteristics and oligoethylenimine (OEI) conjugated via an aminoacrylate (AA) linker that can be cleaved by reactive oxygen species (ROS). In aqueous media, the polymer could self‐assemble into bright red fluorescent nanoparticles (NPs), which can efficiently bind to DNA through electrostatic interaction for gene delivery. Upon visible light irradiation, the generated ROS can break the endo/lysosomal membrane and the polymer, resulting in light‐controlled endo/lysosomal escape and unpacking of DNA for efficient gene delivery. The smart polymer represents the first successful gene vector to simultaneously address both challenges with a single light excitation process.
Durch eine einzige Bestrahlung mit Licht kann ein photoaktiver polymerer Genvektor aus dem Endo/Lysosom entweichen und Nukleinsäuren ins Zytosol abgeben. Das Polymer besteht aus einem Fluorogen mit aggregationsverstärkter Emission und einem Linker, der durch reaktive Sauerstoffspezies gespalten wird. Es bildet Nanopartikel, die endozytotisch aufgenommen werden und an DNA binden.
Url:
DOI: 10.1002/ange.201503640
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001D46
- to stream Istex, to step Curation: 001D46
- to stream Istex, to step Checkpoint: 000130
- to stream Main, to step Merge: 000F86
- to stream Main, to step Curation: 000F85
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Photoactivatable AIE Polymer for Light‐Controlled Gene Delivery: Concurrent Endo/Lysosomal Escape and DNA Unpacking</title>
<author><name sortKey="Yuan, Youyong" sort="Yuan, Youyong" uniqKey="Yuan Y" first="Youyong" last="Yuan">Youyong Yuan</name>
</author>
<author><name sortKey="Zhang, Chong Ing" sort="Zhang, Chong Ing" uniqKey="Zhang C" first="Chong-Jing" last="Zhang">Chong-Jing Zhang</name>
</author>
<author><name sortKey="Liu, Bin" sort="Liu, Bin" uniqKey="Liu B" first="Bin" last="Liu">Bin Liu</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:851C87152357537B753BBA9406626131473D55EF</idno>
<date when="2015" year="2015">2015</date>
<idno type="doi">10.1002/ange.201503640</idno>
<idno type="url">https://api.istex.fr/ark:/67375/WNG-QDCHCHRQ-5/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001D46</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001D46</idno>
<idno type="wicri:Area/Istex/Curation">001D46</idno>
<idno type="wicri:Area/Istex/Checkpoint">000130</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000130</idno>
<idno type="wicri:doubleKey">0044-8249:2015:Yuan Y:a:photoactivatable:aie</idno>
<idno type="wicri:Area/Main/Merge">000F86</idno>
<idno type="wicri:Area/Main/Curation">000F85</idno>
<idno type="wicri:Area/Main/Exploration">000F85</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">A Photoactivatable AIE Polymer for Light‐Controlled Gene Delivery: Concurrent Endo/Lysosomal Escape and DNA Unpacking</title>
<author><name sortKey="Yuan, Youyong" sort="Yuan, Youyong" uniqKey="Yuan Y" first="Youyong" last="Yuan">Youyong Yuan</name>
<affiliation wicri:level="4"><country xml:lang="fr">Singapour</country>
<wicri:regionArea>Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive</wicri:regionArea>
<orgName type="university">Université nationale de Singapour</orgName>
</affiliation>
</author>
<author><name sortKey="Zhang, Chong Ing" sort="Zhang, Chong Ing" uniqKey="Zhang C" first="Chong-Jing" last="Zhang">Chong-Jing Zhang</name>
<affiliation wicri:level="4"><country xml:lang="fr">Singapour</country>
<wicri:regionArea>Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive</wicri:regionArea>
<orgName type="university">Université nationale de Singapour</orgName>
</affiliation>
</author>
<author><name sortKey="Liu, Bin" sort="Liu, Bin" uniqKey="Liu B" first="Bin" last="Liu">Bin Liu</name>
<affiliation wicri:level="4"><country xml:lang="fr">Singapour</country>
<wicri:regionArea>Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive</wicri:regionArea>
<orgName type="university">Université nationale de Singapour</orgName>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Singapour</country>
<wicri:regionArea>Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 3 Research Link, Singapore</wicri:regionArea>
<wicri:noRegion>Singapore</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Singapour</country>
</affiliation>
<affiliation wicri:level="4"><country xml:lang="fr">Singapour</country>
<wicri:regionArea>Correspondence address: Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive</wicri:regionArea>
<orgName type="university">Université nationale de Singapour</orgName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Angewandte Chemie</title>
<title level="j" type="sub">Internationales Jahr des Lichts</title>
<title level="j" type="alt">ANGEWANDTE CHEMIE</title>
<idno type="ISSN">0044-8249</idno>
<idno type="eISSN">1521-3757</idno>
<imprint><biblScope unit="vol">127</biblScope>
<biblScope unit="issue">39</biblScope>
<biblScope unit="page" from="11581">11581</biblScope>
<biblScope unit="page" to="11585">11585</biblScope>
<biblScope unit="page-count">5</biblScope>
<publisher>WILEY‐VCH Verlag</publisher>
<pubPlace>Weinheim</pubPlace>
<date type="published" when="2015-09-21">2015-09-21</date>
</imprint>
<idno type="ISSN">0044-8249</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0044-8249</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Endo/lysosomal escape of gene vectors and the subsequent unpacking of nucleic acids in cytosol are two major challenges for efficient gene delivery. Herein, we report a polymeric gene delivery vector, which consists of a photosensitizer (PS) with aggregation‐induced emission (AIE) characteristics and oligoethylenimine (OEI) conjugated via an aminoacrylate (AA) linker that can be cleaved by reactive oxygen species (ROS). In aqueous media, the polymer could self‐assemble into bright red fluorescent nanoparticles (NPs), which can efficiently bind to DNA through electrostatic interaction for gene delivery. Upon visible light irradiation, the generated ROS can break the endo/lysosomal membrane and the polymer, resulting in light‐controlled endo/lysosomal escape and unpacking of DNA for efficient gene delivery. The smart polymer represents the first successful gene vector to simultaneously address both challenges with a single light excitation process.</div>
<div type="abstract" xml:lang="de">Durch eine einzige Bestrahlung mit Licht kann ein photoaktiver polymerer Genvektor aus dem Endo/Lysosom entweichen und Nukleinsäuren ins Zytosol abgeben. Das Polymer besteht aus einem Fluorogen mit aggregationsverstärkter Emission und einem Linker, der durch reaktive Sauerstoffspezies gespalten wird. Es bildet Nanopartikel, die endozytotisch aufgenommen werden und an DNA binden.</div>
</front>
</TEI>
<affiliations><list><country><li>Singapour</li>
</country>
<orgName><li>Université nationale de Singapour</li>
</orgName>
</list>
<tree><country name="Singapour"><noRegion><name sortKey="Yuan, Youyong" sort="Yuan, Youyong" uniqKey="Yuan Y" first="Youyong" last="Yuan">Youyong Yuan</name>
</noRegion>
<name sortKey="Liu, Bin" sort="Liu, Bin" uniqKey="Liu B" first="Bin" last="Liu">Bin Liu</name>
<name sortKey="Liu, Bin" sort="Liu, Bin" uniqKey="Liu B" first="Bin" last="Liu">Bin Liu</name>
<name sortKey="Liu, Bin" sort="Liu, Bin" uniqKey="Liu B" first="Bin" last="Liu">Bin Liu</name>
<name sortKey="Liu, Bin" sort="Liu, Bin" uniqKey="Liu B" first="Bin" last="Liu">Bin Liu</name>
<name sortKey="Zhang, Chong Ing" sort="Zhang, Chong Ing" uniqKey="Zhang C" first="Chong-Jing" last="Zhang">Chong-Jing Zhang</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000F85 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000F85 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= ChloroquineV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:851C87152357537B753BBA9406626131473D55EF |texte= A Photoactivatable AIE Polymer for Light‐Controlled Gene Delivery: Concurrent Endo/Lysosomal Escape and DNA Unpacking }}
This area was generated with Dilib version V0.6.33. |